Effects of microbial fermentation on enzyme activity and volatile properties of <i>Massa Medicata Fermentata</i>

Aim Massa Medicata Fementata (MMF) is a crude drug used in East Asia to treat anorexia and dyspepsia. It prepared from wheat several herbs through microbial fermentation using Aspergillus sp. Rhizopus There great difference the quality of commercial MMF, microbes MMF are suggested affect its quality. We investigated effects on MMF. Methods Raw materials were mixed according ratio listed National Standard for Chinese Patent Drugs, was pure cultures oryzae or oryzae. Digestive enzyme activities (α-amylase, protease, lipase) volatile compounds measured an analytical kit GC–MS, respectively. Results Enzyme activity increased with A. (MMF-A) showed higher α-amylase lipase than that R. (MMF-R). Protease marginally MMF-R MMF-A. GC–MS analysis revealed terpenoids decreased fermentation; however, 2,3-butanediol, acetoin, guaiacol detected only. C8 such as 1-octen-3-ol MMF-A MMF-R; aromatic 4-vinylguaiacol pyrazines Conclusion Microbial contributes changes volatiles. These properties considerably affected by used, it proposed this study important have control production